- 作者: 王三郎; 張文智; 呂明洲
- 作者服務機構: 大葉工學院食品工程系
- 中文摘要:
利用蒸煮粉碎處理過之蝦蟹殼粉作為篩選耐鹼性幾丁質?生產菌之基質。菌株K-187具較佳之酵素生產性。此菌株
經鑑定係為Pseudomonas aeruginosa。於含3%蝦蟹殼粉,0.1 % CMC,0.1 % (NHa ) S0 ,0.1 % K HP0 ,0.1 %MgS0
‧7H O,以及0.1%ZnS0 (pH9)之培養基,經三天培養(45℃)可得較佳之酵素活性。酵素反應之最適pH與溫度分別
為7及40℃,而pH及熱穩定性則係分別為5-10及60℃。
告 - 英文摘要: Shrimp and crab shell powder prepared by treating shrimp and crab processing waste with boilingand crashing was used as a substrate for isolating alkali-tolerant chitinolytic microorganisms. Strain K-187appeared to be the chitinase-producing strain with the most potential. The organism was identified as astrain of Pseudomonas aeruginosa. Maximum chitinase activity was obtained when the strain was grownaerobically in a medium consisting of 3.0% shrimp and crab shell powder, 0.1% CMC, 0.1%(NH4)2S04,0.1% K2HP04, 0.1% MgS04.D7H20 and 0.1% ZnS04 (pH 9), at 45℃ after 3 days. The optimum pHand temperature of the enzyme reaction were 7 and 40℃, respectively. The chitinase was stable at pHfrom 5 to 10 and was stable under 60℃.
- 中文關鍵字: chitinase; chitin; shrimp and crab shell powder; seafood processing waste; Pseudomonas aeruginosa.
- 英文關鍵字: --