- 作者: Chih-Chien Tsai; Chun-Li Chen; Hwa-Chung Liu; Yi-Ting Lee; Hsei-Wei Wang; Lein-Tuan Hou; Shih-Chieh Hung
- 作者服務機構: Stem Cell Laboratory, Department of Medical Research & Education and Orthopaedics & Traumatology, Veterans General Hospital, Taipei, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
To overcome loss of stem-like properties and spontaneous differentiation those hinder the
expansion and application of human mesenchymal stem cells (hMSCs), we have clonally
isolated permanent and stable human MSC lines by ectopic overexpression of primary
cell cultures of hMSCs with HPV 16 E6E7 and human telomerase reverse transcriptase
(hTERT) genes. These cells were found to have a differentiation potential far beyond the
ordinary hMSCs. They expressed trophoectoderm and germline specific markers upon
differentiation with BMP4 and retinoic acid, respectively. Furthermore, they displayed
higher osteogenic and neural differentiation efficiency than primary hMSCs or hMSCs
expressed HPV16 E6E7 alone with a decrease in methylation level as proven by a global
CpG island methylation profile analysis. Notably, the demethylated CpG islands were
highly associated with development and differentiation associated genes. Principal
component analysis further pointed out the expression profile of the cells converged
toward embryonic stem cells. These data demonstrate these cells not only are a useful
tool for the studies of cell differentiation both for the mesenchymal and neurogenic
lineages, but also provide a valuable source of cells for cell therapy studies in animal
models of skeletal and neurological disorders. - 中文關鍵字: --
- 英文關鍵字: --