- 作者: Kimberly D. Brewer, Genevieve M. Weir, Iulia Dude, Christa Davis, Cathryn Parsons, Andrea Penwell, Rajkannan Rajagopalan, Leeladhar Sammatur, Chris V. Bowen and Marianne M. Stanford
- 作者服務機構: 1. Biomedical Translational Imaging Centre (BIOTIC), Halifax, Canada 2. Department of Diagnostic Radiology, Dalhousie University, Halifax, Canada 3. Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Canada 4. Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada 5. School of Biomedical Engineering, Dalhousie University, Halifax, Canada 6. Immunovaccine Inc., Halifax, Canada
- 中文摘要:
- 英文摘要:
Background
Oil emulsions are commonly used as vaccine delivery platforms to facilitate slow release of antigen by forming a depot at the injection site. Antigen is trapped in the aqueous phase and as the emulsion degrades in vivo the antigen is passively released. DepoVax™ is a unique oil based delivery system that directly suspends the vaccine components in the oil diluent that forces immune cells to actively take up components from the formulation in the absence of passive release. The aim of this study was to use magnetic resonance imaging (MRI) with additional biological markers to evaluate and understand differences in clearance between several different delivery systems used in peptide-based cancer vaccines.
Methods
C57BL/6 mice were implanted with a cervical cancer model and vaccinated 5 days post-implant with either DepoVax (DPX), a water-in-oil emulsion (w/o), a squalene oil-in-water emulsion (squal o/w) or a saponin/liposome emulsion (sap/lip) containing iron oxide-labeled targeted antigen. MRI was then used to monitor antigen clearance, the site of injection, tumour and inguinal lymph node volumes and other gross anatomical changes. HLA-A2 transgenic mice were also vaccinated to evaluate immune responses of human directed peptides.
Results
We demonstrated differences in antigen clearance between DPX and w/o both in regard to how quickly the antigen was cleared and the pattern in which it was cleared. We also found differences in lymph node responses between DPX and both squal o/w and sap/lip.
Conclusions
These studies underline the unique mechanism of action of this clinical stage vaccine delivery system. - 中文關鍵字:
- 英文關鍵字: Magnetic resonance imaging (MRI), Cancer, Vaccines, Emulsion, DepoVax, Biomarker