- 作者: Phil-Sun Oh; Kye-Taek Lim
- 作者服務機構: 1 Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Kwangju City, 500-757, South Korea; ; 2 Molecular Biochemistry Laboratory, Biotechnology Research Institute, Chonnam National University, 300 Yongbong-Dong, Kwangju City, 500-757, South Korea
- 中文摘要: --
- 英文摘要: The Solarium nigrum Linne (SNL) has been traditionally used as a herbal agent in folk medicine for various cancers in Korea. We found that the SNL glycoprotein consists of carbohydrate (69.74%) and protein content (30.26%), which has mainly the hydrophobic amino acids containing glycine and proline. With respect to its characters, we evaluated the apoptotic effects of glycoprotein isolated from SNL in human cervical cancer cell. In the activity of the apoptotic related proteins [cytochrome c, caspase 8, 3 and poly (ADP-ribose) polymerase (PARP)], the results showed that SNL glycoprotein (50 μg/ml) has a stimulatory effect on cytochrome c release into cytosol, caspase 8, 3 activation and PARP cleavage in HeLa cells. To verify the possible mechanism for apoptotic activity of SNL glycoprotein in HeLa cells, the binding activities of transcription factors (NF-κΒ and AP-1) and nitric oxide (NO) production was evaluated. The activities of NF-κΒ and AP-1 significantly decreased after SNL glycoprotein (50 μg/ml) treatment for 4 h, compare to the control. Interestingly, there was no difference of the DNA binding activity between NF-κΒ and AP-1 Also, nitric oxide (NO) production was significantly declined at 50 μg/ ml SNL glycoprotein for 4 h. Therefore, we speculated that SNL glycoprotein exhibits inhibitory effect on HeLa cells via apoptosis, and it may be a potential candidate in field of anticancer drug discovery.
- 中文關鍵字: --
- 英文關鍵字: apoptosis, caspase 3, nitric oxide, SNL glycoprotein, transcription factor