- 作者: Chun-Yi Liau* and Chung-Sheng Lin
- 作者服務機構: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, R.O.C.
- 中文摘要: A non-denatured SDS-PAGE followed by in-gel activity staining using embedded glycol chitin as a substrate was used to identify the proteins with chitinolyitc activities from sweet potato leaf extract. At least two chitinase activity zones can be clearly identified on the gel at positions with estimated molecular weights of 54.1~55.6 kDa and 39.6 kDa. Furthermore, our data also indicate that the activity of the larger one can withstand the standard SDS-PAGE sample preparation. Both of these chitinases, however, are different from that of the previously identified chitinase in sweet potato leaves, which has a molecular weight of 16 kDa. By using an embedded substrate, our method has superior sensitivity in detecting chitinases with higher molecular weights. It is a simple, affordable way and may aid in the future discovery of new chitinases.
- 英文摘要: --
- 中文關鍵字: Chitinase; Glycol chitin; Activity staining; Sweet potato.
- 英文關鍵字: --