- 作者: Daryl E. Warder Michael J. Keherly
- 作者服務機構: Department of Surgery, Divsion of Neurosurgery, University of Texas Medical Branch, Galveston, Tex., USA
- 中文摘要: --
- 英文摘要: The matrin 3 family of nuclear proteins consists of mem-bers with potentially diverse activities. Matrin 3 andNP220 share RNA-binding domains, and NP220 hasbeen shown to recognize and bind to the DNA sequence,CCCCC (G/C). We have isolated and characterized anoth-er member of the matrin 3 family, designated NP94, froma medulloblastoma. This protein, also named Ciz1, haspreviously been characterized for its ability to interactwith p21 and contains 3 zinc finger domains and amatrin 3-homologous domain 3. Our immunofluores-cence and Northern blot analysis data indicate that Ciz1is localized in the nucleus and is expressed in a widerange of tissues, especially the pancreas and the brain;within the brain, the highest message levels are found inThe nucleic acid sequences and deduced amino acid sequences ofCiz1 cDNA clones NP94 and NP94b are available from the GenBankdatabase under GenBank Accession No. 159.025 and GenBankAccession No. 234,161.the cerebellum. A modified selected and amplified bind-ing (SAAB) sequence method was used to identify DNAsequences recognized by Ciz1. From the analysis of theretrieved SAAB sequences and verification using electro-phoretic mobility shift assays, we formulated a consen-sus DNA sequence, ARYSR(0-2)YYAC, recognized byCiz1. The potential activities of Ciz1, including thoseinvolved in brain tumorigenesis, are discussed.
- 中文關鍵字: --
- 英文關鍵字: DNA-binding protein. Matrin 3. Medulloblastoma. p21. Nuclear Protein