• 作者： 呂勝春; 許金玉; 張富雄; 周民治; 楊照雄
• 作者服務機構： 中央研究院生化研究所; 臺灣大學臨床醫學研究所; 行政院衛生署預防醫學研究所; 臺灣大學細菌科
• 中文摘要： 由B型肝炎表面抗原陽性之血漿中純化的B型肝炎Dane顆粒具有蛋白質致活?的效能。這些純化的Dane顆粒於
  100 mM MnCl 和0.5％ NP-40的存在下與【γ P】ATP在30℃培養15秒後由電泳分析法可測到20K，42K，48K，50K及56
  K道爾頓等的磷化多胜。在人類肝癌細胞存在下，Dane顆粒做如上的磷化反應，?無法測到42K，48K，50K道爾頓等
  磷化多胜，如果Dane顆粒在人類周邊血液淋巴球的特別成分存在下也做如上的磷化實驗，則其生成的磷化多胜不變。
  由近期的一些報導，從肝癌組織純化的B型肝炎Core顆粒具蛋白質致活?效能，而其生成的磷化多胜為20K道爾頓。我
  們的實驗顯示Dane顆粒在肝癌細胞（或組織）存在下，其蛋白質致活?只能選擇性的磷化某些多胜。
  氏
• 英文摘要： Protein kinase activity has been found in hepatitis B virions (Dane particles) purified from theplasma of hepatitis B surface antigen carriers [Albin, C., and Robinson, W.S. (1980) J. Virol. 34, 297-302].Dane particles were purified from the pooled, HBeAg-positive plasma. When this preparation was incubatedwith [γ P]ATP in the presence of lOmM MnC1 and 0.5% NP-40 for 15 seconds at 30℃, several pho-sphorylated polypeptides of 20,000, 42,000, 48,000, 50,000 and 56,000 daltons were detected in sodiumdodecyl sulfate-polyacrylamide gels. When the Dane particles were incubated with [γ P]ATP, 10 mMMnC1 , and 0.5% NP-40 in the presence of human hepatoma cell(J-5) particultate fraction at 30℃, 15seconds, the 42,000, 48,000 and 50,000 daltons phosphorylated polypeptides were not found. Whenhuman peripheral blood lymphocytes particulate fraction was incubated with Dane particles under thesame conditions, no change of Dane particle phosphorylated polypeptides was detected. Previous publica-tions [Albin, C.. and Robinson, W.S. (1980) J. Virol. 34, 297-302; Gerlich, W.H. et al. (1982) J. Virol.42, 761-766] showed that when hepatitis B core particles purified from hepatoma tissues contained proteinkinase activity, only phosphorylated polypeptide was 20,000 daltons. Our data suggested that when Daneparticels were put in an environment of hepatoma cells (or tissues), the protein kinase could only pho-sphorylate selected polypeptides in these particles.
• 中文關鍵字： hepatitis B virus; hepatoma cell; protein kinase
• 英文關鍵字： --