• 作者： 王錦梅; 翁祖輝; 林仁混
• 作者服務機構： 國立台灣大學醫學院生化科
• 中文摘要： 我們選擇交通擁擠，車流量大的林森南路地下道研究長短期曝露於高度圬染的空氣下對鼠體解毒酵素及肝、肺組織之影響，空氣懸浮微粒樣品的致突變性及16種polycyclic aromatic hydrocarbons （PAH）的含量也同時評估作為參考，發現經一個月的曝露後，ICR老鼠體重減輕9克，肝臟中細胞色素P-450, benzo （a） pyrene hydroxylase，及肺中nitrore-ductase都有顯著增加且P<0.005，肝中nitroreductase雖有增加但並不顯著，而六個月的曝露期可使曝露組的肺均有肺氣腫、炭末沈著，及慢性發炎等症狀，一隻鼠肝表面有眼可見的白斑，直徑約0.5cm，切片後可見在central vein附近有明顯的組織壞死，及找到核深染細胞。
• 英文摘要： The air in the Lin-Shen tunnel in Taipei is heavily polluted due to heavy traffic. To elucidatethe biologic effects of air pollution, we studied the biochemical alterations of Polycyclic aromatic hy-drocarbons metabolic enzymes and histological changes in the livers and lungs in experimental mice underlong-term and short-term exposure to the tunnel air. The concentrations of 16 Polycyclic aromatichydrocarbons in the air were measured by HPLC, and the mutagenicity of the extract of air particulateswas assayed by the Ames test. We found that the benzo(a)pyrene hydroxylase and cytochrome P-450in the livers and nitroreductase in the lungs of the 30-day exposed animals were significantly increasedas compared to the unexposed control (p＜0.005). The nitroreductase in the liver also increased butnot significantly. The lungs of long-term (6 mo.)exposed mice showed anthracosis, chronic inflammation,and emphysema (10/10). The livers of the experimental animals did not show significant alteration.A whitish circular nodule appeared on the liver surface of one mouse (1∕10). Microscopically, thenodule showed necrosis and hyperchromatic cells around the central vein. It is concluded that the pollutedair in the traffic tunnel has evident biohazardous effects on the lungs and livers of mice under the ex-perimental conditions.
• 中文關鍵字： air pollution; mutagenicity; PAHs metabolic enzymes; histologic changes.
• 英文關鍵字： --