• 作者： 趙祖怡; 朱燦銘
• 作者服務機構： 國防醫學院內科學科; 三軍總醫院內科部血液及腫瘤科; 美國紐約州羅斯威爾派克學院診斷免疫及生化學系
• 中文摘要： 本研究利用BALB/c白鼠對於正常脾臟細胞及以基因重組介白質-2（rIL-2）培養脾細胞生成之淋球素活化殺手（LAK）細胞在靜脈注射後體內之分佈情形做了分析。標記有鉻-51放射性元素之正常脾細胞及LAK細胞在靜脈注射入正常BALB/c 白鼠6,24,及 48 小時後以定位指標（localization index）來分析資料。 LAK 細胞在靜脈注射後主要匯聚於脾，肺，肝，淋巴結，骨骼及腎臟，並有一早期分佈至肺臟之狀況。 LAK 細胞在靜脈注射後6及24小時分佈至肺臟之狀況並未見於正常脾細胞。因為幾乎有三分之一的 LAK 細胞會羈留於脾臟，因此脾臟切除術對於 LAK 細胞在體內之移行應有所影響。基於以上原因，我們對於 LAK 細胞在脾臟切除之BALB/c白鼠體內之分佈做了進一步分析。結果顯示脾臟切除術可增加 LAK 細胞匯聚至肺，肝，淋巴結及骨骼之程度。因此，脾臟切除術或許可以增加 LAK 細胞對存於這些器官之轉移性癌症之治療效果。
• 英文摘要： The in vivo distribution of intravenously injected lymphokine activated killer (LAK) cells, generatedin vitro with rIL-2 from normal murine splenocytes, was studied in BALB/c mice and compared with thator normal splenocytes. Both normal splenocytes and LAK cells were labeled with Cr, and the resultswere analyzed at 6, 24, and 48 hours after injection by localization index as the parameter. After in-jection through tail veins of mice, LAK cells were found to migrate to the spleen, lungs, liver, lymph nodes,bones and the kidneys. The apparent increased distribution pattern of LAK cells to the lung at 6 and 24hours after injection was not detected when normal splenocytes were injected. Since almost one third ofthe injected LAK cells were found to localize in the spleen, it was postulated that splenectomy would affectthe in vivo organ distribution of LAK cells. Accordingly, the in vivo distribution of LAK cells in splenec-tomized mice was further investigated. Results indicated that splenectomy enhanced the convergence ofLAK cells to the lungs, liver, lymph nodes and bones. Therefore, splenectomy may augment the therapeu-tic effect of the adoptive transfer of LAK cells in pulmonary，hepatic, lymph node and bony metastases.
• 中文關鍵字： lymphokine activated killer cells; splenectomy
• 英文關鍵字： --