- 作者: 謝維澤
- 作者服務機構: 國立臺灣大學生化科學研究所
- 中文摘要:
麩酸醱酵後的廢液含有菌體、殘糖、殘氨和代謝物,嚴重污染環境。為減少廢液引起之污染,吾人收回菌體,利用
菌體生產核?酸。從細菌體抽出核酸,用酵素水解核酸,用色層分析法分離核?酸。再用電泳法,紫外線光譜和核?酸
水解?檢定產物為5’腺?酸,5’尿?酸,5’胞?酸和5’烏?酸。
a - 英文摘要: Corynebacterium glutamicum cells are industrially used for glutamate production. However, thewaste that contains microbial cells, cellular debris, residual sugars, ammonia and metabolites seriously pol-lutes the environment. The cells are recovered and utilized for ribonucleotide production so that the pol-lution caused by the cells is eliminated. Nucleic acid is extracted from the cells and is hydrolyzed withnuclease PI from Penicillium citrinum. The hydrolysate is fractionated with Dowex-50 and Dowex-1 into5'-CMP, 5'-UMP, 5'-GMP and 5'-AMP. The products are characterized by electrophoresis, ultraviolet lightabsorbance, and 5'-nucleotidase.
- 中文關鍵字: Cornebacterium glutamicum; glutamate; ribonucleotides
- 英文關鍵字: --