- 作者: 朱曉蘭, 朱慶生, 高芸
- 作者服務機構: Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P. R. China
- 中文摘要: A fast, simple, and sensitive HPLC method for the determination of free amino acids in tobacco was described. A fully automated sample processor performed precolumn derivatization of both primary and secondary amino acids with o-phthalaldehyde/3-mercaptopropionic acid and 9-fluorenylmethyl chloroformate (FMOC-Cl), respectively. All reactions were fully automated by means of an injector programme and accomplished in 10 min. Sample preparation consisted of a single step of extraction with 0.1 mol/L HCl at ambient temperature (assisted by sonication) in 30 min, followed by filtration of an aliquot and derivatization. By optimization of sample preparation and HPLC conditions, separation of 20 amino acids in 30 min was achieved. Detection limits ranged from 0.50 to 1.40μg/g; coefficients of variation ranged from 1.8% to 3.9%; recoveries ranged from 84.6% to 108.5%. The method was applied to the analysis of amino acids contents of tobacco leaves in different varieties and flue-curing period.
- 英文摘要: --
- 中文關鍵字: Amino acids; Tobacco; Column liquid chromatography; Fluorescence detection; Pre-column derivatization.
- 英文關鍵字: --