• 作者： Chen, Shu-Jen; Cheng, Chu-Yuan; Chen, Teh-Liang
• 中文摘要： 本文探討Acinetobacter radioresistens以正十六烷為碳源，發酵生產脂肪酵素。此菌株需要一段很長的適應期才能開始利用正十六烷生長，而且脂肪酵素的生產速率很慢。將正十六烷乳化或誘導細胞表面親油性的產生，都能增加細胞的生長速率，也因而增加脂肪酵素的生產速率。乳化正十六烷可經由乳化劑的添加，誘導細胞表面親油性則可藉著在正十六烷的存在下培養接種液而得到。將這兩種操作合併使用，脂肪酵素產量可達155U/mL，而發酵所需時間為54小時。與控制組實驗比較，脂肪酵素產量增加了3.5倍，而發酵時間縮短了66小時。另外，本文也闡述了正十六烷、油酸、乳化劑、細胞表面親油性在此脂肪酵素生產中所扮演的角色。
• 英文摘要： Lipase production by Acinetobacter radioresistens was investigated using n-hexadecane as the carbon source. A long lag time was required for the cells to grow on n-hexadecane, and the lipase production rate was slow. Both emulsification of n-hexadecane and induction of cell-surface hydrophobicity increased cell growth rate and thus the lipase production rate. The former was achieved by adding emulsifiers to the medium, while the latter was obtained by growing the inoculum on n-hexadecane. A combination of both operations resulted in a lipase yield of 155U/mL and the fermentation time required was 54h. Compared with the control run, the lipase yield was increased by 3.5-fold and the fermentation time was shortened by 66h. The roles of n-hexadecane, oleic acid, emulsifiers and cell-surface hydrophobicity in the lipase production were elucidated.
• 中文關鍵字： 脂肪酵素; 正十六烷; 細胞表面親油性
• 英文關鍵字： Lipase; Acinetobacter Radioresistens; N-Hexadecane; Cell-Surface Hydrophobicity