• 作者： 林耀輝；張光遠
• 作者服務機構： 中央研究院植物研究所
• 中文摘要： 從綠豆芽抽出的粗染色質之放射性NAD+聚合產物，除了緩衝液洗滌外，如果不經其他抽取過程，可以?酸或強鹼處 理，使它成?可溶於百分之五的三氯乙酸。Pronase ，胰?的RNase A ，分別對上述聚合產物的溶解性有一些影響。胰臟的 DNase I和蛇毒的phosphodiesterase則沒有影響。大?有百分之五十的上述聚合產物可溶於中性的NH2OH 。這上層液如 果以濾紙層次分析法檢驗可以看出五個部分。其中三個分別相當於ADPR, 5'-AMP和adenosine 。另外兩個是未知'A' 和'B' 。 'A'留在原點而'B'則緊接在5'-AMP之前。如果以?鹼抽取上述聚合產物，抽取液經濾紙層次分析則發現：絕大 多數的放射性物質留在原點，而另外?量可觀的放射性物質相當於ADPR。這?鹼抽取液如果再經蛇毒的phosphodiesterase 和pronase處理，然後以濾紙層次分析法檢驗則發現：原來留在原點的放射性物質，幾乎完全消失，同時相當於5'-AMP， 未知物'B'和adenosine'的放射性物質則增加。
• 英文摘要： 14C(Adenine-u)-NAD+-incorporation product(s) of Phaseolus radiatus chromatin was rendered 5% TCA-soluble in situ by acid or base treatment. Pronase and pancreatic RNase A had some and slight effects on unextracted incorporation product(s) respectively. Pancreatic DNase i, snake venom phosphodiesterase had no effect at all. About 50% of the unextracted product(s) was made soluble by neutral NH2OH. This soluble fraction, when analyzed by paper chromatography, contained five subfractions. Three of them corresponded to ADPR, 5'-AMP, and adenosine. The other two were unknowns 'A' and 'B'. 'A' remained at origin and 'B' moved right before 5'-AMP. Of alkaline extract of the incorporation product(s), majority of the radioactive materials remained at origin while a considerable amount had mobility corresponding to ADPR. When alkaline extract was treated further with snake venom phosphodiesterase and pronase treatments, radioactivities at the origin disappeared almost completely with simultaneous increase of radioactivities corresponding to 5'-AMP, unknown 'B', and adenosine.
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