• 作者： 陳貴年; 溫振源; 謝正勇
• 作者服務機構： 國立臺灣大學醫學院解剖學科
• 中文摘要： 本實驗以HRP打入大白鼠咀嚼肌各肌，經逆行性運輸後，標幟出支配咀嚼肌之神經元，以TMB-HRP法標幟之神經元作為光學顯微鏡術研究，而DAB-HRP法標幟之神經元則作電子顯微鏡術研究。實驗結果顯示，將HRP打入閉頜肌後，在三叉神經中腦徑核上具有標幟神經元。而開頜肌中，除HRP打入下頜舌骨肌後，標幟出極少數神經元外，打入外翼肌及二腹肌前腹後，在中腦徑核中未有標幟神經元。支配每一閉頜肌之中腦徑核神經元分散於全核，但稍有集中於核尾側之現象。這些標幟神經元是單極神經元，核圓或橢圓。每一神經元具一突起走向核之後外側，加入三叉神經中腦徑中。超微構造顯示中腦徑核中支配咀嚼肌之神經元細胞核大，形狀規則，位於胞體之正中或偏旁。胞質中具發達之高基氏體及粗糙型內質網。神經細絲成束或分散與高基氏體交雜。胞體表面具棘狀突起，但僅具極少數突觸。二相鄰之胞體間，偶或有類似黏著斑之構造。
• 英文摘要： The neurons innervating the muscles of mastication were labeled retrogradely with horseradishperoxidase (HRP) which was injected into each muscle of mastication of the rats. The TMB-HRP labeledneurons were for light microscopic and DAB-HRP labeled neurons for electron microscopic study. ManyHRP-labeled mesencephalic neurons were observed in the trigeminal mesencephalic nucleus (TMEN) afterHRP injection in jaw-closing muscles (JCM). On the other hand, no labeled neurons were found followingthe application of HRP to the lateral pterygoid and the anterior belly of the digastric muscles, with theexception of a very few from the mylohyoid muscle. The latter three muscles were jaw-opening muscles(JOM). The mesencephalic neurons of each JCM in the TMEN were rather randomly distributed, althoughthey were concentrated more in the caudal region of this nucleus. These neurons were typically unipolar,with spherical to oval perikarya. Each neuron had a single process which coursed caudolaterally to jointhe mesencephalic tract of the trigeminal nerve. Ultrastructurally, mesencephalic masticatory neurons hada rather regular nucleus locating either centrally or eccentrically in the perikaryon, which is rather plump.The cytoplasm was endowed with very well developed Golgi apparatus and rough endoplasmic reticulum.Neurofilaments, varying in number, intermingled mostly with the Golgi apparatus in the cytoplasm.Somatic spines were frequently observed; however, synapses abutting upon the soma were few. Maculaadherens-like structures were ocassionally encountered in the contact zone between two cells.
• 中文關鍵字： trigeminal mesencephalic neurons; masticatory muscles; rat
• 英文關鍵字： --