- 作者: 盧瀗鍹;孫婉美;陳順文;羅銅壁
- 作者服務機構: 國立臺灣大學生化科學研究所; 中央研究院生物化學研究所
- 中文摘要: 比較苯基硫代內醯?(PTH)胺基酸不同水解法,結果以6N鹽酸(含1%硫氫基乙醇及0.2%酚)較其他已知方法 為佳,S-羧甲基半胱胺酸?收率可高至80%以上,而不安定的Thr及Ser也有可靠的收得量。此法可成功地應用在蛋白質及胜?序列分析之鑑定工作。
- 英文摘要: For back hydrolysis of PTH-amino acids, the use of 6N HC1 containing 1% mercaptoethanol and 0.2% phenol was found to be the most satisfactory when compared to any other known procedures. The recovery of S-carboxymethyl-cystein was more than 80% and labile threonine and serine could be recovered in significant amount. The applicability was tested and compared by the sequence analysis of two known peptides.
- 中文關鍵字: --
- 英文關鍵字: --