- 作者: Esther R. Picache; Loubna Hassanieh; Daniel Broek; Axel H.Schonthal
- 作者服務機構: Department of Molecular Microbiology and Immunology, and Molecular Biology and Biochemistry, and Keck School Medicine and K. Norris Jr. Comprehensive Cancer Center, University of Southern California, Los Angeles, Calif., USA
- 中文摘要: --
- 英文摘要: A cross-linked form of the detergent Triton X-100, calledTriton WR-1339, has been shown to reduce the spread oftumor cells in laboratory animals. However, some ofthese effects were controversial,probably due to the useof different tumor cell lines and varying sites of injection.In order to better understand these processes, we haveused Triton X-100 and performed a molecular analysis ofits growth-inhibitory function.Using the T24 bladder car-cinoma cell line, we have shown that treatment of cellswith this detergent caused a potent anti proliferativeeffect resulting from the downregulation of the key cellcycle regulators, the cyclin-dependent kinases(CDKs).CDK activity was lost due to a twofold effect, theincreased expression of the CDK inhibitors p21ciP1 andp27KiP1 in combination with the reduced expression ofcyclin A, a regulatory CDK subunit that is essential forCDK function.Taken together, our results provide a mo-lecular basis for the antiproliferative effects of the Tritondetergent, namely its differential effects on various partsof the cell cycle machinery.
- 中文關鍵字: --
- 英文關鍵字: --