• 作者： 沈弘德; 林文玲; 張曉寧; 黃夢麟; 韓韶華
• 作者服務機構： 台北榮民總醫院醫學研究部及內科部; 國防醫學院醫學系; 國立陽明醫學院微生物及免疫學研究所
• 中文摘要： 青黴菌(Penicillium)普遍存在於室內環境中，為引發氣喘誘因之一。我們的調查結果顯示，P. citrinum為台北地區出現頻率最高之青黴菌。本研究乃以免疫轉印法分析人類血清中IgE及IgG抗體所作用之P. citrinum成分。結果發現共有8種分子量介於112與17.5kD間之成分，可與11位病人血清中之IgE抗體反應。其中33kD成分與IgE抗體呈現反應強與高頻率(81.8%)之反應。此外，共有11種P. citrinum成分可與人類 IgG抗體反應；其中又以分子量約66kD成分與IgG的反應頻率較高(58.3)。在9個有抗33kD成分IgE抗體之病人血清中，僅有3個血清亦具抗此33kD成分之IgG抗體。在來自5位健康實驗室工作人員的血清中，有4個血清中具IgG抗體可與P. citrinum成分反應，但役有一個血清中有與P. citrinum成分反應之IgE抗體存在。本實驗結果顯示，以SDS-PAGE免疫轉印法分析，33kD成分為P. citrinum中最重要之IgE-binding成分。至於對同一種過敏原粗製粹取液呈現不同之IgG與IgE反應所代表的意義，仍需做進一步的探討。
• 英文摘要： Penicillium species are well-known indoor fungi and have been considered as one of the causativeagents of extrinsic bronchial asthma. We have identified that Penicillium citrinum is the most prevalentPenicillium species in the Taipei area. In the present study, components of this airborne fungus recognizedby human IgE and IgG antibodies were analyzed by immunoblotting. Eight components ranging in MWsfrom 112 to 17.5 kD reacted with IgE antibodies in 11 serum samples. The component with an MW ofabout 33 kD showed the highest frequency (81.8%)of IgE-binding. However, 11 components of P. citrinumshowed IgG-immunoblotting activity, and the component with an MW of about 66 kD revealed the highestfrequency (58.3%) of IgG-binding. Only 3 of the 9 serum samples which reflected IgE antibody activityto the 33 kD component of P. citrinum showed IgG-immunoblotting activity to the same 33 kD component.Furthermore, 4 of the 5 serum samples obtained from healthy laboratory personnel demonstrated IgGresponses, but none had IgE antibody activity to components of p. citrinum. The discrepancy observedbetween IgE and IgG responses to the same allergenic extract requires further investigation.
• 中文關鍵字： P.citrinum; IgE-binding components; immunoblotting.
• 英文關鍵字： --