- 作者: 蔡來誠; 林資琛; 吳文麗; 韓韶華; 王豐明; 梁長齡; 袁九重; 陳弘民
- 作者服務機構: 榮民總醫院醫學研究部臨床免疫研究室; 榮民總醫院外科部大腸直腸外科
- 中文摘要: 以腫瘤胚胎抗原(CEA)單源抗體進行人大腸直腸癌之免疫酵素組織染色法定位組織CEA。組織染色呈CEA陽性反應者計22位直腸癌者有20位(陽性率90.9%)及所有23位大腸癌者(100%),6位腺瘤瘜中有2位(33.3%);然4位增殖性瘜肉均呈陰性反應。組織CEA反應在分化良好之腺癌組織往往比分化稍差或不良之腺癌組織強且多。而腸癌組織CEA反應之呈現與疾病型期無關。在組織染色呈陰性、弱及強反應之病人血中CEA含量值分別為 5.4±3.9 ng/ml, 28.3±23.8ng/ml及 99.8±145.3 ng/ml。其中39位組織染色呈強反應者有30位(78.9%)血中CEA含量升高,而6位弱反應及10位陰性反應者中血中CEA含量升高分別為4位(66.7%)及1位(10%)。此組織CEA弱或強陽性反應組均較陰性反應組中血中CEA含量為高,且在統計學上呈有意義之差異(P<0.01)。本結果認為CEA單源抗體(MAb C27)利用免疫酵素組織染色於一般的腸癌組織切片測定CEA,對常規病理之診斷有其可行性。
- 英文摘要: Immunoperoxidase localization of carcinoembryonic antigen (CEA) was performed on tissuesections of colorectal carcinoma using a monoclonal antibody (MAb) against CEA. CEA has been demon-strated in 20 out of 22 rectum carcinomas (90.9%), in all of 23 colonic carcinomas, in none of 4 hyper-plastic polyps and in 2 out of 6 adenomatous polyps (33.3%). CEA was found more often, and the in-tensity of the staining was stronger in well-differentiated carcinomas than in moderately and poorly dif-ferentiated carcinomas. No correlation was found between the presence of CEA in colorectal carcinomaand the stages of the disease. The mean values of serum CEA in patients with colorectal carcinoma andpolyps with negative, weakly and strongly positive staining were 5.4±3.9 ng/ml, 28.3±23.8 ng/ml and99.8±145.3 ng/ml respectively. Elevation of serum CEA occurred in 30 out of 39 (78.9%) cases withstrongly positive CEA staining, in 4 out of 6 (66.7%) with weakly positive and in 1 out 9 (11.1%) withnegative staining. A significant difference was found in serum CEA activity between the group with negativeCEA staining and positive CEA staining (P < 0.01). Our results suggest that the monoclonal antibody(MAb C27) can be used for the localization of CEA in conventionally prepared tissues of colorectal car-cinomas by immunoperoxidase techniques for routine immunopathological diagnosis.
- 中文關鍵字: colorectal carcinoma (CRC); monoclonal antibody (MAb); carcinoembryonic antigen (CEA)
- 英文關鍵字: --