- 作者: Jing-Ruey Yeh; Li-Chung Hsu; Bon-chu Chung
- 作者服務機構: Institute of Molecular Biology, Academia Sinica, Nankang and Institute of Biochemistry, National Yang-Ming University Shin-Pai, Taipei, Taiwan, Republic of China
- 中文摘要: --
- 英文摘要: We characterized a regulatory element located in the -76to -62 region of the human ferredoxin gene. This regionbound to Sp1-like proteins with low affinity, as shownusing electrophoretic mobility shift, competition, anti-body binding, and Southwestern experiments. The simi-larity of the regulatory element to Sp1 extends beyondits DNA-binding domain, as cloned Sp1 functionedequally well when fused to a peptide that bound to anirrelevant site. The function of these Sp1-binding sites ismediated through the cAMP-dependent protein kinase(PKA) signaling pathway, because reporter genes down-stream of the Sp1-binding sites were not activated in aPKA-deficient cell line. Transfection of the catalytic sub-unit of PKA restored activated transcription. Similar Sp1-binding sites identified in the CYP11A1 and CYP21 genesalso controlled cAMP-dependent transcription of the re-porter gene. Our finding of the function of Sp1-like pro-teins in steroidogenic gene transcription adds one morerole Sp1 plays in controlling physiological events.
- 中文關鍵字: --
- 英文關鍵字: --