- 作者: Bin Zeng; Xiao-feng Ren; Feng Cao; Xiao-yang Zhou; Jing Zhang
- 作者服務機構: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, Mainland China
- 中文摘要: --
- 英文摘要:
Background: Although recent studies have highlighted the role of epicardial cells
during cardiac development and regeneration, their cardiomyogenic potential is still
controversial due to the question of lineage tracing of epicardial cells. The present
study therefore aimed to examine the the expression of Tbx18 and Wt1 in embryonic
heart and to identify whether Tbx18 and Wt1 themselves expressed in the
cardiomyocyte.
Methods: Mouse embryonic hearts were collected at different stages for
immunofluorescence costaining with either Tbx18 and the cardiac transcription factor
Nkx2.5 or Wilms tumor 1 (Wt1) and Nkx2.5.
Results: Tbx18 and Wt1, but not Nkx2.5, were expressed in the proepicardium and
epicardium. Tbx18 was expressed in cells within the heart from E10.5 to at least
E14.5; these Tbx18-expressing cells were Nkx2.5 positive, except for a few cells that
were Nkx2.5 negative at E14.5. Wt1 was expressed in cells within the heart from
E12.5 to at least E14.5, but these Wt1-expressing cells were Nkx2.5 negative.
Conclusion: The data obtained in this study demonstrate that Tbx18 is expressed in
the cardiomyocytes from E10.5 to at least E14.5, and Wt1 is expressed within the
heart from E12.5 to at least E14.5, but not in the cardiomyocyte. These findings may
provide new insights on the role of the epicardial cells in cardiac regeneration. - 中文關鍵字: --
- 英文關鍵字: Cardiac progenitor; Epicardial cells; Tbx18; Wt1; Development