- 作者: Chien-Der Lee; Yao-Pei Yan; Shu-Mei Liang; Ting-Fang Wang
- 作者服務機構: 1Institute of Molecular Biology, Academia Sinica, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Virus-like particles (VLPs) are formed by the self-assembly of envelope and/or capsid
proteins from many viruses. Some VLPs have been proven successful as vaccines, and
others have recently found applications as carriers for foreign antigens or as scaffolds in
nanoparticle biotechnology. However, production of VLP was usually impeded due to
low water-solubility of recombinant virus capsid proteins. Previous studies revealed that
virus capsid and envelope proteins were often posttranslationally modified by SUMO in
vivo, leading into a hypothesis that SUMO modification might be a common mechanism
for virus proteins to retain water-solubility or prevent improper self-aggregation before
virus assembly. We then propose a simple approach to produce VLPs of viruses, e.g.,
foot-and-mouth disease virus (FMDV). An improved SUMO fusion protein system we
developed recently was applied to the simultaneous expression of three capsid proteins of
FMDV in E. coli. The three SUMO fusion proteins formed a stable heterotrimeric
complex. Proteolytic removal of SUMO moieties from the ternary complexes resulted in
VLPs with size and shape resembling the authentic FMDV. The method described here
can also apply to produce capsid/envelope protein complexes or VLPs of other diseasecausing
viruses. - 中文關鍵字: --
- 英文關鍵字: --