- 作者: Yuh Ho, Ming-Ching Hsu and Fu-Yung Huang
- 中文摘要: A solution of γ-crystallin became turbid upon heating at 65℃ for 30 minutes; however, addition of α-crystallin suppressed this thermal aggregation. It was found the effective chaperone function could be achieved with the molar ratio of α/γgreater than 1/20. In terms of crystalline subunit, five molecular α-crystallin subunits could afford chaperone for one molecular γ-crystallin. The gel filtration profile of the sample solution, containing α- and γ-crystallins, and preincubation at 65℃ for 30 minutes, showed complex formation between α- and γ-crystallins, indicating α-crystallin was bound to thermally denatured γ-crystallin. A 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence study showed that α-crystallin has more hydrophobic regions exposed after thermal incubation. In the presence of urea, both the α-crystallin chaperone activity and the ANS fluorescence intensity deceased. Accordingly, hydrophobic regions of α-crystallin play an indispensable role in its chaperone activity.
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