- 作者: Pretty Mary Abraham; Korah P Kuruvilla; Jobin Mathew; Anitha Malat; Shilpa Joy; C s Paulose
- 作者服務機構: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Kerala, India
- 中文摘要: --
- 英文摘要:
Diabetes and stress stimulate hippocampal 5-HT synthesis, metabolism and release.
The present study was carried out to find the effects of insulin, Aegle marmelose alone and in
combination with pyridoxine on the hippocampal 5-HT, 5-HT2A receptor subtype, gene
expression studies on 5-HT2A, 5-HTT, INSR, immunohistochemical studies and elevated plus
maze in streptozotocin induced diabetic rats. 5-HT content showed a significant decrease
(p<0.001) and a significant increase (p<0.001) in 5-HIAA in hippocampus of diabetic rats
compared to control. 5-HT receptor binding parameters Bmax and Kd showed a significant
decrease (p<0.001) whereas 5-HT2A receptor binding parameters Bmax showed a significant
decrease (p<0.001) with a significant increase (p<0.05) in Kd in hippocampus of diabetic rats
compared to control. Gene expression studies of 5-HT2A, 5-HTT and INSR in hippocampus
showed a significant down regulation (p<0.001) in diabetic rats compared to control.
Pyridoxine treated in combination with insulin and A. marmelose to diabetic rats reversed the
5-HT content, Bmax , Kd of 5-HT, 5-HT2A and gene expression of 5-HT2A, 5-HTT and INSR in
hippocampus to near control. The gene expression of 5-HT2A and 5-HTT were confirmed by
immunohistochemical studies. Behavioural studies using elevated plus maze showed that
serotonin through its transporter significantly increased (p<0.001) anxiety-related traits in
diabetic rats which were corrected by combination therapy. Our results suggest that
pyridoxine treated in combination with insulin and A. marmelose has a role in the regulation
of insulin synthesis and release, normalising diabetic related stress and anxiety through
hippocampal serotonergic function. This has clinical significance in the management of
diabetes. - 中文關鍵字: --
- 英文關鍵字: --