• 作者： 王三郎; 張文智; 呂明洲
• 作者服務機構： 大葉工學院食品工程系
• 中文摘要： 利用蒸煮粉碎處理過之蝦蟹殼粉作為篩選耐鹼性幾丁質?生產菌之基質。菌株K-187具較佳之酵素生產性。此菌株
  經鑑定係為Pseudomonas aeruginosa。於含3％蝦蟹殼粉，0．1 ％ CMC，0．1 ％ （NHa ） S0 ，0.1 ％ K HP0 ，0．1 ％MgS0
  ‧7H O，以及0.1％ZnS0 （pH9）之培養基，經三天培養（45℃）可得較佳之酵素活性。酵素反應之最適pH與溫度分別
  為7及40℃，而pH及熱穩定性則係分別為5-10及60℃。
  告
• 英文摘要： Shrimp and crab shell powder prepared by treating shrimp and crab processing waste with boilingand crashing was used as a substrate for isolating alkali-tolerant chitinolytic microorganisms. Strain K-187appeared to be the chitinase-producing strain with the most potential. The organism was identified as astrain of Pseudomonas aeruginosa. Maximum chitinase activity was obtained when the strain was grownaerobically in a medium consisting of 3.0％ shrimp and crab shell powder, 0.1％ CMC, 0.1％(NH4)2S04,0.1％ K2HP04, 0.1％ MgS04.D7H20 and 0.1％ ZnS04 (pH 9), at 45℃ after 3 days. The optimum pHand temperature of the enzyme reaction were 7 and 40℃, respectively. The chitinase was stable at pHfrom 5 to 10 and was stable under 60℃．
• 中文關鍵字： chitinase; chitin; shrimp and crab shell powder; seafood processing waste; Pseudomonas aeruginosa.
• 英文關鍵字： --