- 作者: 萬家茂; 張瑩珠; 黃麗芸; 廖欽峰; 余玉林
- 作者服務機構: 中央研究院動物研究所內分泌實驗室; 國立臺灣大學醫學院生理科
- 中文摘要:
為了解腦垂腺在大量分泌及合成促甲素之情況下所發生各種功能之改變,必須建立一情況,使腦垂腺在不受激素?
饋及代謝物之影響下持續分泌及合成促甲素。本文報告乃以周流法用甲釋素持續刺激可得擬似體內甲狀腺功能過低時之
大鼠腦下腺,以此一情況,可用於了解,促甲素分泌細胞高度致活後腦垂腺各功能之改變。p - 英文摘要: The production of immunoreactive TSH by hemipituitaries (Hps) to the stimulation of TRH inperifusion with negligible influence of the feedback of secretogogues and hormones was analysed. Thestimulations were of long term continuous (3 hrs) and short term (15 min), with the dose levels of 1, 10and 100 ng per ml of medium (for 3 hrs) or in 2 ml (for 15 min). The largest amount in production and thefastest rate in release of TSH found in present report is at 10 ng level. Only total TSH, in tissue plus inmedium, after continuous TRH stimulation were dose-related, but not in release alone. We present hereinan analysis of pituitary TSH production, in a perifusion system under steady TRH stimulation. Thisarrangement is believed to be a condition simulating hypothyroidism in pituitary level and suitable forstudy of the functions of the pituitary with hyperactivated thyrotrophs.
- 中文關鍵字: in vitro perifusion; rat pituitary; simulatory hypothyroidism; thyrotropin; thyrotropin releasing hormone
- 英文關鍵字: --