• 作者： 簡靜香; 張國廷; 周松男
• 作者服務機構： 國立陽明醫學院生化學科; 國立臺灣大學醫學院附屬醫院婦產科
• 中文摘要： 對人類卵巢腺癌之十二個檢體，卵巢癌腹水細胞，及NIH：OVCAR-3細胞株，作 c-Ki-ras 致癌基因之檢測結果，四個檢體，腹水細胞及細胞株皆具有 c-Ki-ras 之擴大。與人類纖維細胞及正常卵巢組織比較，此 c-Ki-ras 致癌基因擴大之現象與腫瘤之期數成正相關，其RNA transcripts及 p21蛋白產物亦增加。五個腫瘤檢體具有 c-Ki-ras 之活化，即 RNA之表現增加，但不具有基因擴大之現象。 此檢測成果，可提供人類卵巢癌之致癌機制之訊息。
• 英文摘要： The amplification and expression of the cellular oncogene c-Ki-ras have been detected in twelvetissue specimens of human ovarian adenocarcinoma, one ascites of ovarian cancer and ovarian cancer cellline NIH: OVCAR-3. Four out of the twelve cancer tissue specimens, one specimen of cancer ascites cellsand the NIH: OVCAR-3 cancer cell line showed elevated amplification of c-Ki-ras, as compared to thehuman fibroblast cell line FS4 and normal ovarian tissues. The amplification of the c-Ki-ras gene showedsome correlation with clinical stages of ovarian cancer. The levels of c-Ki-ras specific RNA transcripts and ras gene product p21 were also elevated in thesefour cancer tissue specimens. Surprisingly, a discrepancy between amplification and expression of c-Ki-raswas noted in five of the other eight cancer tissue specimens, in which enhanced expression of c-Ki-raswas present without the accompanying elevated amplification of c-Ki-ras. The results do provide some clues to understanding the possible mechanisms of tumorigenesis inhuman ovarian cancer.
• 中文關鍵字： gene amplification; gene expression; c-Ki-ras ovarian cancer; human; oncogene
• 英文關鍵字： --