• 作者： 曾銘仁；陳水田；翁?惠；王光燦
• 作者服務機構： 中央研究院生物化學研究所; 國立臺灣大學生化科學研究所
• 中文摘要： 利用Z-Phe-Leu-OTMB為模式，研究pH，反應時間，基質濃度和酵素濃度各種變數對木瓜酵素催化胜?鍵形成的影響，由Z-Phe和Leu-OTMB合成此化合物的最佳條件為63 mM的基質和25 mg/ml的酵素溶於pH6.0的0.2M McIlvaine緩衝液和乙醇(7:5 v/v)的混合液。於38°C下反應，6小時後，得純化Z-Phe-Leu-OTMB 92%的產率。
• 英文摘要： Using Z-Phe-Leu-OTMB as the model compound, the papain-catalyzed peptide bond formation was studied by varying the parameters including pH, reaction time, and concentrations of substrate and enzyme. Optimal yield of the model compound was obtained when Z-Phe and Leu-OTMB were reacted in 0.2 M Mcllvaine buffer-ethanol (7:5 v/v), pH 6.0, at 38蚓 for 6hrs with substrate concentration of 63 mM and enzyme concentration of 25 mg/ml. Under this condition, highly purified Z-Phe-Leu-OTMB was prepared in 92% yield.
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