• 作者： 薛敬和;徐添財;熊光濱;何橈宜
• 作者服務機構： 國立清華大學化學工程研究所;國立陽明醫學院醫學工程研究所;工業技術研究院化學工業研究所;榮民總醫院內科部新陳代謝科
• 中文摘要： 葡萄糖分析儀之原型機已試製完成，並且已證明有應用於臨床檢驗之實用價值。本分析儀是以葡萄糖酵素電極即Clark型。 溶氧電極，配合固定化酵素薄膜組合而成。固定化酵素薄膜係以1.5－戊雙醛或Carbodiimide兩種共價結合固定法，固定於醋酸纖維素系之Cuprophane　薄膜表面而成。 本研究試製之分析儀可分析葡萄糖濃度範圍為10至300 mg/dl，所需之檢體體積約10微升至0.5毫升之問，分析時間若以應答時間計算僅須20秒，每一酵素薄膜可重覆分析至少360個檢體而不會降低活性。分析之誤差百分比小於百分之一。因此，若酵素薄膜之穩定性可繼續的研究改進，則本分析儀應可實際應用於臨床上葡萄糖之分析。
• 英文摘要： A glucose analyzer has been constructed which has the potential of clinical laboratory application.
  The analyzer is made of a glucose enzyme electrode and necessary electronic circuits.
  The Qlucose enzyme electrode is made of a Clark-tyne oxyen electrode covered with an enzyme
  membrane wmcn is preparea by immobinzmg glucose oxiaase onto a CupropnaneR membrane by eitner the
  glutaraldehyde or the carbodiimide method.
  The analyzer can be used to analyze glucose within a range of 10-300 mg/dl. The sample volumes
  ranged from 10 microliter to 0.5 mililiter. The response time is 20 seconds determined by the kinetic meth-
  od. Each enzyme membrane can be used repetitively for at least 360 times without any loss of its activity.
  The ％ error of serum glucose determination is less than 1%. Therefore, if the stability of the enzyme mem-
  brane can be further improved, the analyzer can be used practically in a clinical laboratory for glucose
  determination.
  a
• 中文關鍵字： biosensor; glucose analyzer; glucose oxidase; enzyme immobilization; Cuprophane membrane
• 英文關鍵字： --