- 作者： Himangshu Sonowal1, Atul Kumar1, Jina Bhattacharyya2, Pabitra Kumar Gogoi2 and Bithiah Grace Jaganathan
- 作者服務機構： Stem Cell and Cancer Biology Group, Indian Institute of Technology Guwahati, Guwahati, Assam, India
- 中文摘要： --
- 英文摘要：
Background: Mesenchymal Stem Cells (MSC) are important candidates for therapeutic applications due to their ex vivo proliferation and differentiation capacity. MSC differentiation is controlled by both intrinsic and extrinsic factors and actin cytoskeleton plays a major role in the event. In the current study, we tried to understand the initial molecular mechanisms and pathways that regulate the differentiation of MSC into osteocytes or adipocytes.
Results: We observed that actin modification was important during differentiation and differentially regulated during adipogenesis and osteogenesis. Initial disruption of actin polymerization reduced further differentiation of MSC into osteocytes and osteogenic differentiation was accompanied by increase in ERK1/2 and p38 MAPK phosphorylation. However, only p38 MAPK phosphorylation was down regulated upon inhibition of actin polymerization which as accompanied by decreased CD49E expression.
Conclusion: Taken together, our results show that actin modification is a pre-requisite for MSC differentiation into osteocytes and adipocytes and osteogenic differentiation is regulated through p38 MAPK phosphorylation. Thus by modifying their cytoskeleton the differentiation potential of MSC could be controlled which might have important implications for tissue repair and regeneration. - 中文關鍵字： --
- 英文關鍵字： Actin remodeling; MSC differentiation; Integrin CD49E
