• 作者： 蔡輝彥
• 作者服務機構： 中國醫藥學院 藥理學科
• 中文摘要： 本實驗係在探討一些具神經活性的多胜物質對於大白鼠脊髓中五羥色胺釋放之影響，以及研究這些神經多胜物質在脊髓中與serotonergic neurons之關係。結果顯示：（1） Somatostatin、substance P和疼痛刺激均會引起脊髓中五羥色胺的釋放；但neurotensin、β-endorphin和met-enkephalin則無影響。（2） Somatostatin所致的五羥色胺釋放之作用，在整體動物中發現可被baclofen和met-enkephalin所抑制（3） Substance P所致之五羥色胺的釋放作用可被baclofen有意義的抑制，而且在整體動物中可被met-enkephalin所加強（4）疼痛刺激所致的五羥色胺之釋放作用可被met-enkephalin和baclofen所抑制，但是此釋放作用可被naloxone加強。由以上之結果，進一步的證明神經多胜物質在中樞所負的重要角色；而且由高級中樞下行的serotonergic neurons在脊髓中之釋放，可能受到含神經多胜物質的中間神經原所調整。
• 英文摘要： The effects of neuroactive peptides on the release of 5-HT were studied. The 5-HT released from thespinal cord was significantly increased by somatostatin, substance P and peripheral pain stimulation (tailpinch), but not affected by neurotensin, β-endorphin and met-enkephalin. The somatostatin-evoked 5-HTrelease was inhibited by baclofen and met-enkephalin in vivo but not in vitro. The substance P-evoked5-HT release was strongly inhibited by baclofen, and slightly potentiated by met-enkephalin in vivo but notin vitro. The tail pinch-induced 5-HT release was inhibited by met-enkephalin and baclofen, but potent-iated by naloxone. These findings provide further evidence on the important role of neuropeptides and suggest thatthe descending serotonergic neurones are modulated by neuropeptide interneurones in the spinal cord.
• 中文關鍵字： neuroactive peptides; 5-hydroxytryptamine; spinal cord
• 英文關鍵字： --