- 作者: Nandhu M S; Jes Paul; Korah P K; Anitha Malat; Chinthu Romeo; C S Paulose
- 作者服務機構: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Kerala, India
- 中文摘要: --
- 英文摘要:
Parkinson’s disease is characterized by progressive cell death in the substantia
nigra pars compacta, which leads to dopamine depletion in the striatum and indirectly to
cortical dysfunction. Increased glutamatergic transmission in the basal ganglia is
implicated in the pathophysiology of Parkinson’s disease and glutamate receptor
mediated excitotoxicity has been suggested to be one of the possible causes of the
neuronal degeneration. In the present study, the effects of serotonin, gammaaminobutyric
acid and bone marrow cells infused intranigrally to substantia nigra
individually and in combination on unilateral 6-hydroxydopamine induced Parkinson’s
rat model was analyzed. Scatchard analysis of total glutamate and NMDA receptor
binding parameters showed a significant increase in Bmax (P<0.001) in the cerebral cortex
of 6-hydroxydopamine infused rat compared to control. Real Time PCR amplification of
NMDA2B, mGluR5, bax, and ubiquitin carboxy-terminal hydrolase were up regulated in
cerebral cortex of 6-hydroxydopamine infused rats compared to control. Gene expression
studies of GLAST, ά-Synuclien and Cyclic AMP response element-binding protein
showed a significant (P<0.001) down regulation in 6-OHDA infused rats compared to
control. Behavioural studies were carried out to confirm the biochemical and molecular
studies. Serotonin and GABA along with bone marrow cells in combination showed
reversal of glutamate receptors and behaviour abnormality shown in the Parkinson’s rat
model. The therapeutic significance in Parkinson’s disease is of prominence. - 中文關鍵字: --
- 英文關鍵字: --