- 作者: Chia-Chu Tsai; Kai-Wen Huang; Hsiao-Fen Chen; Bo-Wen Zhan; Yen-Han Lai; Fa-han Lee; Chung-Yei Lin; Yi-Cheng Ho; Yu-Wei Chao; Yih-Ching Su; Sen-Wen Jane; Yu-Chi Chen; Chyong-Ing Hsu; Po-Huang Li; Hey-Chi Hsu; Yutaka Suzuki; Sumio Sugano; Jung-Yaw Lin
- 作者服務機構: 1 Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan, R.O.C; 2 Department of Surgery, National Taiwan University Hospital, Taipei, 100, Taiwan, R.O.C; 3 Department of Pathology and Graduate Institute of Pathology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan, R.O.C; 4 Laboratory of Genome Structure Analysis, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
- 中文摘要: --
- 英文摘要: Hepatocellular carcinoma (HCC) is a leading cause of death worldwide. Hepatitis B virus (HBV) or hepatitis C virus (HCV) infection has been shown to cause hepatic carcinogenesis. A total 58,251 of cDNA clones of full-length cDNA libraries of HBV and HCV-infected HCC and their surrounding non-tumor tissues, respectively, were sequenced and analyzed by blasting against GENEBANK maintained by NCBI. About 180 and 279 of genes were shown an obviously increased and decreased expression patterns between HCC tissue and its adjacent non-tumor tissue. The candidate genes consisted of the genes encoded liver specific metabolism enzymes, secretory functional proteins, proteases and their inhibitors, protein chaperon, cell cycle components, apoptosis-related proteins, transcriptional factors, and DNA binding proteins. Several genes were further investigated by using real-time PCR to confirm the gene expression levels in at least 24 pairs of HCC tissues and adjacent non-tumor tissues. The results showed that genes encoded reticulon 4, RGS-1, antiplasmin, and kallikrein B were down-regulated with the average of 2.8, 8.5, 3.2, and 10.5-fold, respectively. Our results provide crucial candidate genes to develop clinical diagnosis and gene therapy of HCC
- 中文關鍵字: --
- 英文關鍵字: full-length cDNA library, gene expression, HCC, real-time PCR, transcriptome