- 作者: 賴峻森
- 作者服務機構: Contribution from the Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, China
- 中文摘要: A radiochemical method using 14C-labeld N-ethylmaleimide (NEM-14C) as a reagent for sulfhydryl determination is reassessed and described in detail. The method is based on the activity of 14C-labeled S-succinyl-L-cysteine (III-14C) recovered from the controlled hydrolysis of the NEM-14C treated sulfhydryl-containing peptide and the correlation between III-14C and SH content established with glutathione. It is simple, accurate and highly sensitive and is not affected by disulfide group as indicated by experiments with insulin. Good result is obtained from tests with β-lactoglobulin. It gives a value of 1.94 mole SH per mole protein, which is close to that generally accepted.
- 英文摘要: --
- 中文關鍵字: --
- 英文關鍵字: --