- 作者: Wen-Jen Lu; Ikumi Tamai; Jun-ichi Nezu; Ming-Liang Lai; Jin-ding Huang
- 作者服務機構: 1 Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; ; 2 Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; ; 3 Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan; ; 4 Chugai Pharmaceutical Co., Ltd., Ibaraki, Japan; ; 5 Department of Neurology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; ; 6 Department of Pharmacology, Medical College, National Cheng Kung University, 1 University Road, Tainan, 70101, Taiwan
- 中文摘要: --
- 英文摘要: Arsenic is an established human carcinogen. The role of aquaglyroporins (AQPs) in arsenic disposition was recently identified. In order to examine whether organic anion transporting polypeptide-C (OATP-C) also plays a role in arsenic transport, OATP-C cDNA was transfected into cells of a human embryonic kidney cell line (HEK-293). Transfection increased uptake of the model OATP-C substrate, estradiol- 17β-D-glu-curonide, by 10-fold. In addition, we measured uptake and cytotoxicity of arsenate, arsenite, monome-thylarsonate(MMAv), and dimethylarsinate (DMAv). Transfection of OATP-C increased uptake and cytotoxicity of arsenate and arsenite, but not of MMAv or DMAv. Rifampin and taurocholic acid (a substrate of OATP-C) reversed the increased toxicity of arsenate and arsenite seen in OATP-C-transfected cells. The increase in uptake of inorganic arsenic was not as great as that of estradiol-17β-D-glucuronide. Our results suggest that OATP-C can transport inorganic arsenic in a (GSH)-dependent manner. However, this may not be the major pathway for arsenic transport.
- 中文關鍵字: --
- 英文關鍵字: arsenic, OATP-C, transporter