- 作者: Peter Dickie
- 作者服務機構: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious; Diseases, National Institutes of Health, Bethesda, Md., USA
- 中文摘要: --
- 英文摘要: The likelihood that expression of a foreign gene in a mammalian cell is deleterious to viability is confronted whenever novel transgenic animals are made. A pathological response to trans- gene expression is even desired in transgenic mouse models of human disease. The derivation of HIV-transgenic mice in our laboratory using multiple recombinant forms of an HIV provi- rus has resulted in mixed success best explained by the variable toxicity of the different trans- genes. Employing a standardized approach to pronuclear injections, experimental variation amongst recombinant HIV transgenes was documented in terms of the percentage of preg- nancies following embryo transfer into pseudopregnant mice and the percentage of trans- planted embryos leading to term births in these pregnant females (giving rise to an index of birth success, SI). Results compiled over 5 years suggested that the SI reflected transgene toxicity, in this case of HIV gene products early in embryogenesis. These observations have guided the design of productive transgenes for mouse models of HIV-related diseases and may be generally applicable in transgenesis.
- 中文關鍵字: --
- 英文關鍵字: Transgene; Mice; Embryogenesis; HIV; Toxicity