第7卷‧第1期,
198301
, pp. 27-31
利用親和力色析法快速純化乳酸脫氫?
- 作者：
張維懋; 林仁混; 張承晉; 熊光濱
- 作者服務機構：
台灣大學醫學生化研究所; 聯合工業研究所應用生物化學研究室
- 中文摘要：
本研究利用Procion dye與Sepharose 4B的共價連結物來純化乳酸脫氫?(LDH)，在所研究的11種 Procion dye之
中，Blue MX-3G被證明能?吸附大量的LDH，而且能用0.3M的KCl沖洗出來，回收率為90%，用此種方法純化所
得的LDH純度很高，在電冰膠上呈現四條異構?電泳帶，純化的LDH在水溶液中可被Blue MX-3G抑制，活性迅速
降低，此種抑制作用可被應用於LDH的純化。
定
- 英文摘要：
Dye-ligand chromatography was examined as a method for the purification of lactate dehydrogenase(LDH, E.C. 1.1.1.27）from rat liver. Eleven dye-sepharose gels were assessed for their ability to bind therat liver LDH. Among eleven Procion dyes used for ligand formation, Procion Blue MX-3G was found to bethe most effective in enabling the column to adsorb and desorb rat liver LDH. The enzyme was purifiedemploying Procion Blue MX-3Gsepharose-4B affinity chromatography to 615-fold with 90% of the totalactivity recovered. The purified enzyme exhibited four enzymatically active protein bands when elec-trophoresised on polyacrylamide gels. Procion Blue MX-3G was found to irreversibly inactivate rat liverLDH in a time-dependent fashion. The result was interpreted in terms of the ability of LDH to bind Pro-cion dyes.
- 中文關鍵字：
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- 英文關鍵字：
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