- 作者: Emmanuelle Lagrue; Hiroyuki Abe; Madakasira Lavanya; Jawida Touhami; Sylvie Bodard; Sylvie Chalon; Jean-Luc Battini; Marc Sitbon; Pierre Castelnau
- 作者服務機構: UMR Inserm U 930, CNRS FRE 2448, Universite Francois Rabelais de Tours, Tours, France
- 中文摘要: --
- 英文摘要:
The gibbon ape leukemia virus (GALV), the amphotropic murine leukemia virus (AMLV) and
the human T-cell leukemia virus (HTLV) are retroviruses that specifically bind nutrient
transporters with their envelope glycoproteins (Env) when entering host cells. Here, we used
tagged ligands derived from GALV, AMLV, and HTLV Env to monitor the distribution of their
cognate receptors, the inorganic phosphate transporters PiT1 and PiT2, and the glucose
transporter GLUT1, respectively, in basal conditions and after acute energy deficiency. For this
purpose, we monitored changes in the distribution of PiT1, PiT2 and GLUT1 in the cerebellum,
the frontal cortex, the corpus callosum, the striatum and the substantia nigra (SN) of C57/BL6
mice after administration of 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridinium (MPTP), a
mitochondrial complex I inhibitor which induces neuronal degeneration in the striato-nigral
network.
The PiT1 ligand stained oligodendrocytes in the corpus callosum and showed a reticular pattern
in the SN. The PiT2 ligand stained particularly the cerebellar Purkinje cells, while GLUT1
labelling was mainly observed throughout the cortex, basal ganglia and cerebellar gray matter.
Interestingly, unlike GLUT1 and PiT2 distributions which did not appear to be modified by
MPTP intoxication, PiT1 immunostaining seemed to be more extended in the SN. The
plausible reasons for this change following acute energy stress are discussed.
These new ligands therefore constitute new metabolic markers which should help to unravel
cellular adaptations to a wide variety of normal and pathologic conditions and to determine the
role of specific nutrient transporters in tissue homeostasis. - 中文關鍵字: --
- 英文關鍵字: --