- 作者: Jung-Chung Lin; Shuen-Kuei Liao; En-Huei Lee; Man-Shan Hung; Yiyang Sayion; Hung-Chang Chen; Chen-Chen Kang; Liang-Sheng Huang; Jaw-Ming Cherng
- 作者服務機構: Laboratory Branch, Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia, U. S. A.
- 中文摘要: --
- 英文摘要:
Epithelial-mesenchymal transition (EMT) is an important process in tumor metastasis.
The EMT-related events associated with metastasis of NPC in the absence of EBV have
not been elucidated. We established an EBV-negative NPC cell line from a bone marrow
biopsy of an NPC patient. Using a Matrigel system we isolated an invasive and noninvasive
sublines, designated NPC-BM29 and NPC-BM00. NPC-BM29 acquired an
invasive-like phenotype characterized by EMT, marked by down-regulation of Ecadherin
and β-catenin with concomitant increased expression of Ets1. NPC-BM29 cells
expressed 10-fold higher of MMP-9 than NPC-BM00 cells. NPC-BM29 cells grew
better in 2% serum than NPC-BM00 cells, with a population doubling-time of 26.8 h and
30.7 h, respectively. A marked reduction in colony-formation ability of NPC-BM00 cells
compared to NPC-BM29 was observed. Wound-healing assay revealed that NPC-BM29
cells displayed higher motility than NPC-BM00 and the motility was further enhanced by
cell treatment with TPA, a PKC activator. Cell surface markers and tumor-associated
molecules, AE3, MAK6 and sialyl-Tn, were up-regulated in NPC-BM29 cells, whereas
the expression of HLA-DR and CD54 was significantly increased in NPC-BM00 cells.
NPC-BM29 consistently released higher levels of IL-8 and IL-10 than NPC-BM00, with
low levels of IL-1 expression in both cell lines. Higher level of VEGF production was
detected in NPC-BM00 than NPC-BM29 cells. These data show that EBV is not required
for exhibiting multiple metastatic phenotypes associated with EMT. More studies that
target right molecules/signalings associated with the EMT may offer new therapeutic
intervention options for NPC invasion and metastasis. - 中文關鍵字: --
- 英文關鍵字: --