- 作者: Jyong-Huei Lee, Kuan-Lun Ho and Shih-Kang Fan
- 作者服務機構: 1. Department of Mechanical Engineering, National Taiwan University, Taipei, Taiwan 2. Department of Mechanical and Nuclear Engineering, Kansas State University, Manhattan, KS, 66506, USA
- 中文摘要:
- 英文摘要:
Engineering approaches were adopted for liver microsystems to recapitulate cell arrangements and culture microenvironments in vivo for sensitive, high-throughput and biomimetic drug screening. This review introduces liver microsystems in vitro for drug hepatotoxicity, drug-drug interactions, metabolic function and enzyme induction, based on cell micropatterning, hydrogel biofabrication and microfluidic perfusion. The engineered microsystems provide varied microenvironments for cell culture that feature cell coculture with non-parenchymal cells, in a heterogeneous extracellular matrix and under controllable perfusion. The engineering methods described include cell micropatterning with soft lithography and dielectrophoresis, hydrogel biofabrication with photolithography, micromolding and 3D bioprinting, and microfluidic perfusion with endothelial-like structures and gradient generators. We discuss the major challenges and trends of liver microsystems to study drug response in vitro. - 中文關鍵字:
- 英文關鍵字: Engineered liver microsystems, Drug response, Cell micropatterning, Hydrogel biofabrication, Microfluidic perfusion