- 作者: Haejeong Lee, Juyoun Shin, Yeun-Jun Chung, Myungseo Park, Kyeong Jin Kang, Jin Yang Baek, Dongwoo Shin, Doo Ryeon Chung, Kyong Ran Peck, Jae-Hoon Song and Kwan Soo Ko
- 作者服務機構: 1. Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea 2. Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea 3. Precision Medicine Research Center, Integrated Research Center for Genome Polymorphism, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea 4. Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, 16419, South Korea 5. Department of Anatomy and Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea 6. Asia Pacific Foundation for Infectious Diseases (APFID), Seoul, 06351, Republic of Korea 7. Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea 8. Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 06351, Republic of Korea
- 中文摘要:
- 英文摘要:
Background
Bacterial isolates with multiple plasmids harbouring different carbapenemase genes have emerged and been identified repeatedly, despite a general notion that plasmids confer fitness cost in bacterial host. In this study, we investigated the effects of plasmids with carbapenemase genes on the fitness and virulence of bacteria.
Methods
Different plasmids harbouring the carbapenemase genes, blaNDM-1 and blaOXA-232, were isolated from a carbapenem-resistant K. pneumoniae strain. Each plasmid was conjugated into the Escherichia coli strain DH5α, and a transconjugant with both plasmids was also obtained by transformation. Their in vitro competitive ability, biofilm formation, serum resistance, survival ability within macrophage and fruit fly, and fly killing ability were evaluated.
Results
The transconjugants with a single plasmid showed identical phenotypes to the plasmid-free strain, except that they decreased fly survival after infection. However, significantly increased fitness, virulence and biofilm production were observed consistently for the transconjugant with both plasmids, harbouring blaNDM-1 and blaOXA-232.
Conclusions
Our data indicate that bacteria carrying multiple plasmids encoding different carbapenemases may have increased fitness and virulence, emphasizing the need for diverse strategies to combat antimicrobial resistance. - 中文關鍵字:
- 英文關鍵字: Carbapenemase, NDM-1, OXA-232, Plasmid, Plasmid paradox