- 作者: 彭志綱; 諾斯;尼生蓋; 邁克;萊文; 艾恩斯;伯恩勤
- 作者服務機構: 國防醫學院牙醫學系牙周病科‧三軍總醫院臨床研究中心; 水牛城紐約州立大學牙周病系 微生物學系 口腔生物學系 皮膚病系
- 中文摘要:
本實驗的目的係以間接免疫螢光法、混合免疫螢光法與免疫吸咐法,去比較三種不同抗基底膜抗體的特異性:(1)實
驗室製備的對抗第四型膠質蛋白抗體,(2)類天?瘡症患者血清中對抗基底膜抗體與(3)大?性表皮鬆解症患者血清中對
抗基底膜抗體。實驗結果顯示三種不同抗體皆與基底膜起反應,但是三種不同抗體作用在不同的基底膜抗原。做免疫吸
咐試驗,發現第一、二、三、或四型膠質蛋白不能降低類天?瘡症與大?表面鬆解症患者血清中對抗基底膜抗體效價與
反應。因此,這些對抗基底膜抗體可以用來探討皮膚病與粘膜病(包括牙周病)基底膜病變的研究。o - 英文摘要: The basement membrane antigenic specificities of antibodies to Type IV collagen were compared tonaturally occurring antibodies in sera from patients with bullous pemphigoid and epidermolysis bullosaacquisita (EBA) by indirect immunofluorescence, mixed immunofluorescence and immunoabsorption. Re-sults suggested that the three sera reacted with three different basement membrane antigens. In addition,absorption with Types Ⅰ, Ⅱ, Ⅲ, or Ⅳ collagen failed to reduce the basement membrane reactivities of bul-lous pemphigoid or EBA sera. The antibodies to the basement membrane components should be useful instudying skin and mucous membrane diseases including periodontal diseases.
- 中文關鍵字: basement membrane; bullous pemphigoid; collagen; epidermolysis bullosa acquisita; indirect immunofluorescence
- 英文關鍵字: --