- 作者: Wei-Hsin Chen, Ching-Liang Hsieh, Chun-Ping Huang, Tzu-Jou Lin, Jason T.C. Tzen, Tin-Yun Ho and Yi-Wen Lin
- 作者服務機構: College of Agriculture and Natural Resources, Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: Peripheral tissue inflammation initiates hyperalgesia accompanied by tissue acidosis,
nociceptor activation, and inflammation mediators. Recent studies have suggested a
significantly increased expression of acid-sensing ion channel 3 (ASIC3) in both
carrageenan- and complete Freund's adjuvant (CFA)-induced inflammation. This
study tested the hypothesis that acupuncture is curative for mechanical hyperalgesia
induced by peripheral inflammation.
Methods:Here we used mechanical stimuli to assess behavioral responses in paw and muscle
inflammation induced by carrageenan or CFA. We also used immunohistochemistry
staining and western blot methodology to evaluate the expression of ASIC3 in dorsal
root ganglion (DRG) neurons.
Results: In comparison with the control, the inflammation group showed significant
mechanical hyperalgesia with both intraplantar carrageenan and CFA-induced
inflammation. Interestingly, both carrageenan- and CFA-induced hyperalgesia were
accompanied by ASIC3 up-regulation in DRG neurons. Furthermore,
electroacupuncture (EA) at the ST36 rescued mechanical hyperalgesia through
down-regulation of ASIC3 overexpression in both carrageenan- and CFA-induced
inflammation.
Conclusions: In addition, electrical stimulation at the ST36 acupoint can relieve mechanical
hyperalgesia by attenuating ASIC3 overexpression. - 中文關鍵字: --
- 英文關鍵字: --