- 作者: Rima Zein-Eddine, Guislaine Refrégier, Jorge Cervantes & Noemí Kaoru Yokobori
- 作者服務機構: 1.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina 2.Laboratoire d’Optique et Biosciences (LOB), Ecole Polytechnique, Route de Saclay 91120, Palaiseau, France 3.Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX, 79905, USA 4.Servicio de Micobacterias, Instituto Nacional de Enfermedades Infecciosas (INEI)-ANLIS and CONICET, C1282AFF, Buenos Aires, Argentina 5.Université Paris-Saclay, CNRS, AgroParisTech, Ecologie Systématique et Evolution, 91190, Gif-Sur-Yvette, France
- 中文摘要:
- 英文摘要:
Clustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems rapidly raised from a bacterial genetic
curiosity to the most popular tool for genetic modifcations which revolutionized the study of microbial physiology.
Due to the highly conserved nature of the CRISPR locus in Mycobacterium tuberculosis, the etiological agent of one of
the deadliest infectious diseases globally, initially, little attention was paid to its CRISPR locus, other than as a phylo‑
genetic marker. Recent research shows that M. tuberculosis has a partially functional Type III CRISPR, which provides
a defense mechanism against foreign genetic elements mediated by the ancillary RNAse Csm6. With the advent of
CRISPR-Cas based gene edition technologies, our possibilities to explore the biology of M. tuberculosis and its interac‑
tion with the host immune system are boosted. CRISPR-based diagnostic methods can lower the detection threshold
to femtomolar levels, which could contribute to the diagnosis of the still elusive paucibacillary and extrapulmonary
tuberculosis cases. In addition, one-pot and point-of-care tests are under development, and future challenges are
discussed. We present in this literature review the potential and actual impact of CRISPR-Cas research on human
tuberculosis understanding and management. Altogether, the CRISPR-revolution will revitalize the fght against tuber‑
culosis with more research and technological developments. - 中文關鍵字:
- 英文關鍵字: CRISPR-Cas, Mycobacterium tuberculosis, MTBC, Spoligotyping, CRISPRi, Functional genomics, Diagnostic methods