• 作者： 趙壯飛; 陳正繹; 曾月貌; 丁令白
• 作者服務機構： 國防醫學院生物及解剖學科; 陽明醫學院微生物及免疫學研究所
• 中文摘要： 本實驗乃應用三種肝癌標示?Glucose-6-phosphotase, gamma-glutamyl transpeptidase及ATPase以生化定量及細胞
  化學方法探討三種分化程度不同之人類肝癌細胞株Hep G2, Hep 3B及HA 22T之反應與細胞分化關係。結果顯示
  Glucose-6-Phosphotase及gamma-glutamyl transpeptidase之活性與肝癌細胞分化程度有關，而ATPase之活性與分化無
  關。依本實驗所得之結果似乎可利用glucose-6-phosphotase及gamma-glutamyl transpeptidas此二?之生化定量及細胞化
  學反應作為臨床肝癌鑑別診斷之標示。之
• 英文摘要： Three enzyme makers, glucose-6-phosphatase, ATPase and gamma-glutamyl transpeptidase, have beenused in studying carcinogenesis of hepatocellular carcinoma. They have been investigated in animal modelsand human hepatocellular carcinoma in vivo and in vitro. But the inconsistent levels of these three enzymesassociated with this type of carcinoma raised the possibility that the carcinoma cells might have derivedfrom the cells originating from different stages of differentiation. To evaluate this possibility, three humancell lines, Hep G2, Hep 3B, and HA 22T, all thought to be arrested in different stages of differentiationbased on their biochemical and morphological characteristics, were used as models. The three enzymemarkers glucose-6-phosphatase, ATPase and gamma-glutamyl transpetidase were examined cytochemicallyand biochemically. Our results showed that there was no correlation between the ATPase levels and thestages of the cell line's differentiation. But both glucose-6-phosphatase and gamma-glutamyl-transpeptidasewere higher in cells that were more differentiated.
• 中文關鍵字： enzyem markers; G-6-Pase; ATPase; Gamma-GTase; human hepatoma cell lines
• 英文關鍵字： --