- 作者: Ling-fang He; Ting-ting Wang; Qian-ying Gao; Guang-feng Zhao; Ya-hong Huang; Li-ke Yu; Ya-yi Hou
- 作者服務機構: Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Mainland China
- 中文摘要: --
- 英文摘要:
Background: Stanniocalcin-1(STC-1) is up-regulated in several cancers including gastric cancer.
Evidences suggest that STC-1 is associated with carcinogenesis and angiogenic process. However,
it is unclear on the exact role for STC-1 in inducing angiogenesis and tumorigeneisis.
Method: BGC/STC cells (high-expression of STC-1) and BGC/shSTC cells (low- expression of
STC-1) were constructed to investigate the effect of STC-1 on the xenograft tumor growth and
angiogenesis in vitro and in vivo. ELISA assay was used to detect the expression of vascular
endothelial growth factor (VEGF) in the supernatants. Neutralizing antibody was used to inhibit
VEGF expression in supernatants. The expression of phosphorylated -PKCβⅡ, phosphorylated
–ERK1/2 and phosphorylated -P38 in the BGC treated with STC-1protein was detected by
western blot.
Results: STC-1 could promote angiogenesis in vitro and in vivo, and the angiogenesis was
consistent with VEGF expression in vitro. Inhibition of VEGF expression in supernatants with
neutralizing antibody markedly abolished angiogenesis induced by STC-1 in vitro. The process of
STC-1-regulated VEGF expression was mediated via PKCβⅡ and ERK1/2.
Conclusions: STC-1 promotes the expression of VEGF depended on the activation of PKCβⅡ
and ERK1/2 pathways. VEGF subsequently enhances tumor angiogenesis which in turn promotes
the gastric tumor growth. - 中文關鍵字: --
- 英文關鍵字: STC-1; angiogenesis; VEGF; PKCβⅡ; ERK1/2.