- 作者: Jia-Wen Wu, Jin-Town Wang, Tzu-Lung Lin, Ya-Zhu Liu, Lii-Tzu Wu & Yi-Jiun Pan
- 作者服務機構: 1.Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan 2.Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan 3.Department of Microbiology and Immunology, School of Medicine, College of Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung, Taiwan 4.Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan 5.Graduate Institute of Biomedical Sciences, College of Medicine, China Medical University, Taichung, Taiwan
- 中文摘要:
- 英文摘要:
Background Klebsiella pneumoniae capsular types K1, K2, K5, K20, K54, and K57 are prevalent hypervirulent types
associated with community infections, and worrisomely, hypervirulent strains that acquired drug resistance have
been found. In the search for alternative therapeutics, studies have been conducted on phages that infect K. pneumoniae K1, K2, K5, and K57-type strains and their phage-encoded depolymerases. However, phages targeting K. pneumoniae K20-type strains and capsule depolymerases capable of digesting K20-type capsules have rarely been reported.
In this study, we characterized a phage that can infect K. pneumoniae K20-type strains, phage vB_KpnM‐20.
Methods A phage was isolated from sewage water in Taipei, Taiwan, its genome was analyzed, and its predicted
capsule depolymerases were expressed and purifed. The host specifcity and capsule-digesting activity of the capsule
depolymerases were determined. The therapeutic efect of the depolymerase targeting K. pneumoniae K20-type
strains was analyzed in a mouse infection model.
Results The isolated Klebsiella phage, vB_KpnM‐20, infects K. pneumoniae K7, K20, and K27-type strains. Three capsule
depolymerases, K7dep, K20dep, and K27dep, encoded by the phage were specifc to K7, K20, and K27-type capsules,
respectively. K20dep also recognized Escherichia coli K30-type capsule, which is highly similar to K. pneumoniae K20type. The survival of K. pneumoniae K20-type-infected mice was increased following administration of K20dep.
Conclusions The potential of capsule depolymerase K20dep for the treatment of K. pneumoniae infections was
revealed using an in vivo infection model. In addition, K7dep, K20dep, and K27dep capsule depolymerases could be
used for K. pneumoniae capsular typing. - 中文關鍵字:
- 英文關鍵字: Klebsiella pneumoniae, Hypervirulent, Phage, Capsule depolymerase