- 作者: Hyo-Soon Jeong; Seung Hoon Lee; Hye-Young Yun; Kwang Jin Baek; Nyoun Soo Kwon; Kyoung-Chan Park; Dong-Seok Kim
- 作者服務機構: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul, Republic of Korea
- 中文摘要: --
- 英文摘要:
Background: Sphingosylphosphorylcholine (SPC) acts as a potent lipid mediator and
signaling molecule in various cell types. In the present study, we investigated the effects of
SPC on melanogenesis and SPC-modulated signaling pathways related to melanin synthesis.
Methods: Melanin production was measured in Mel-Ab cells. A luciferase assay was used to
detect transcriptional activity of the MITF promoter. Western blot analysis was performed to
examine SPC-induced signaling pathways.
Results: SPC produced significant hypopigmentation effects in a dose-dependent manner. It
was found that SPC induced not only activation of Akt but also stimulation of mTOR, a
downstream mediator of the Akt signaling pathway. Moreover, SPC decreased the levels of
LC3 II, which is known to be regulated by mTOR. Treatment with the mTOR inhibitor
rapamycin eliminated decreases in melanin and LC3 II levels by SPC. Furthermore, we found
that the Akt inhibitor LY294002 restored SPC-mediated downregulation of LC3 II and
inhibited the activation of mTOR by SPC.
Conclusions: Our data suggest that the mTOR signaling pathway is involved in SPC-modulated melanin synthesis. - 中文關鍵字: --
- 英文關鍵字: Akt/ LC3 II/Melanocytes/mTOR/sphingosylphosphorylcholine