- 作者: Ednildes de Almeida Olympio Rua1, Marcella Leite Porto2, Jean Pierre Louzada, Breno Valentim Nogueira, Silvana dos Santos Meyrelles, Elisardo Corral Vasquez , Thiago de Melo Costa Pereira
- 作者服務機構: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Av. Comiss rio Jos Dantas de Melo, Boa Vista, Vila Velha, ES,Brazil
- 中文摘要: --
- 英文摘要:
Background:
Although cigarette smoke is known to be a complex mixture of over 4000 substances that can lead to damage through active or passive smoking, its mechanisms and biochemical consequences in pregnancy and neonates are not yet fully understood. Therefore, in the present study, we propose to study the impact of smoking during gestation on the viability of blood mononuclear cells (MNC) from umbilical cords of newborns to assess the degree of oxidative stress and cell viability. Moreover, we aim to investigate possible biochemical and morphological changes in the umbilical cord.
Methods:
After childbirth, the cord blood and the umbilical cord were immediately collected in public hospitals in Greater Vitoria, ES, Brazil. Flow cytometry was used to analyze the cord blood and biochemical and histological tests were used to analyze the umbilical cord.
Results:
Pregnant smokers had a reduction of MNC viability from the umbilical cord (10%), an increase in the production of reactive oxygen species (ROS) and an increase in cell apoptosis (~2-fold) compared to pregnant non-smokers. In the umbilical cord, it was observed an increase of advanced oxidation protein products - AOPP (~2.5-fold) and a loss of the typical architecture and disposition of endothelial cells from the umbilical artery.
Conclusions:
These data suggest that maternal cigarette smoking during pregnancy (even in small amounts)may compromise the viability of MNC cells and damage the umbilical cord structure,possibly by excessive ROS bioavailability. - 中文關鍵字: --
- 英文關鍵字: Cigarette smoke, Cord blood, Oxidative stress, Pregnant women, Apoptosis