- 作者: 江煜勳; 羅平
- 作者服務機構: 國立清華大學輻射生物研究中心
- 中文摘要: 本研究之主題在探討加熱處理及冷凍對V79組織培養細胞之影響。高溫對細胞之存活率之影響與其他報告無異:43℃以下連續加熱使細胞產生耐熱性;加熱時,細胞成附著予培養皿上,成處在懸浮態中,其熱敏感度均相似。雖在懸浮態時加熱,培養液之pH會高達8.2,但其對細胞存活並無影響。如在加熱前先給予細胞冷凍-解凍一次,細胞之存活形成與單獨加熱並無差異。將41到45℃間細胞熱敏感度對溫度作Arrhinius圖結果為一直線,活化能(Activation En-ergy)為171 kcal/mole;其直線在42.5℃並無顯示偏折現象。如細胞在加熱後再給予冷凍-解凍處理,其存活率比以冷凍及加熱為兩獨立現象所計算之存活率為低。
- 英文摘要: The survival rate of V79 Chinese hamster lung fibroblasts treated with heat and freezing was investi-gated. The hyperthermic behavior of these cells was similar to other reports; Thermotolerance was observedbelow 43℃ during continuous heating. There was no difference in survival rate whether the cells wereheated while attached to tissue culture flasks or in suspension, although the pH of the medium during thelatter situation could be around 8.2 during hyperthermic treatment. If the cells were frozen and thawedbefore heating, the pattern of survival did not change significantly from that of heating alone, and ther-motolerance was still observed below 43℃. The Arrhinius plot of heat sensitivities between 41 and 45℃demonstrated a straight line, giving an activation energy of 171 kcal/mole. A break on the plot around41.2℃ could not be locates, probably sue to the lack of experimental points. It the cells were frozen andthawed immediately after heating, the survival rate was lower than that expected from considering hyper-thermia and freezing as two independent processes. Possible explanations are provided in the result anddiscussion section.
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