- 作者: 邱式鴻; Naoyuki ohtsu
- 作者服務機構: 國立臺灣大學生化科學研究所; Linus Pauling Institute of Science and Medicine Palo Alto,California, U.S.A.
- 中文摘要: 抗壞血酸(維生素C)在高濃度下(大於100μM)能夠抑制神經癌(neuroblastoma)和子宮內膜癌(endometrial carci-noma)的生長,但對於正常的肺纖維母細胞(lung fibroblasts)的影響卻很小。抗壞血酸的抗癌作用,在銅離子及其錯合物的存在下,能雊大大的提高。本報告是針對此種抗癌作用,研究它的基本化學機轉模式。抗壞血酸在銅離子或其錯合物的配合下,確和一般抗癌藥物一樣,能診切割細胞的基因分子(DNA),但其抑制癌細胞的主要步驟可能是引起外層細胞膜的破裂。
- 英文摘要: L-Ascorbic acid inhibits the growth of mouse neuroblastoma and human endometrial carcinoma cellsat concentrations greater than 100μM. Under the same concentrations used in cell culture study, normalhuman lung fibroblasts show less sensitivity to the antiproliferative effect of ascorbate than tumor cell lines.The antitumor activity of ascorbate can be greatly potentiated by the combination with copper ions orcopper chelates. The exposure of normal and tumor cells to the mixtures of ascorbate and copper chelates,especially Cu -o-phenanthroline and Cu -2,9-dimethyl-o-phenanthroline complexes, resulted in the kil-ling of a large proportion of cell populations whereas the organic ligand portion of metal complexes wasmuch less toxic. These copper chelates in combination with ascorbate showed different degrees of DNA-scission activities which could not be correlated with their cytotoxicities in the cell culture study. It issuggested that the primary targets of these antiproliferative agents may be on the biological sites such ascell membrane other than DNA in the nucleus which has been commonly assumed as the critical target formost free radical-generating antitumor drugs.
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